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  • 產品名稱:Avidity

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Avidity帶有AviTag標簽的人類全長ORF克隆已經被構建在多種T7和CMV啟動子表達系統,可以立即購買使用。AviTag標簽蛋白可以被體內或體外的生物素連接酶生物素化,而且抗生物素蛋白或鏈霉親和素可以專一地與生物素結合,正基于這兩個反應,AviTag?技術可以應用于蛋白的固定,純化和顯影。 雖然Avidity是一個小公司,科學帶來的好處及其AviTag已得到認可。目前, AviTag技術已經在22個國家的世界十大制藥公司和研究人員中得到認可。
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Avidity

  


Avidity帶有AviTag標簽的人類全長ORF克隆已經被構建在多種T7和CMV啟動子表達系統,可以立即購買使用。AviTag標簽蛋白可以被體內或體外的生物素連接酶生物素化,而且抗生物素蛋白或鏈霉親和素可以專一地與生物素結合,正基于這兩個反應,AviTag?技術可以應用于蛋白的固定,純化和顯影。 雖然Avidity是一個小公司,科學帶來的好處及其AviTag已得到認可。目前, AviTag技術已經在22個國家的世界十大制藥公司和研究人員中得到認可。 



Avidity Avitag? Technology

Avidity develops and sells molecular affinity tools for connecting molecules. Our patented AviTag? technology employs a highly targeted enzymatic conjugation of a single biotin on a unique 15 amino acid peptide tag using the biotin ligase (BirA) from E. coli. AviTag? sequence is GLNDIFEAQKIEWHE. Oriented on streptavidin-coated surfACES, this creates an ideal presentation for molecular binding interactions.

The scientific benefits of Avidity's AviTag Technology have garnered notice. Currently, AviTag technology is licensed by seven of the world's top ten pharmaceutical companies and is used by researchers in 22 countries.

Company History

Avidity, LLC was founded in 1996 by Millard Cull, Larry Lansing, Dr. Ron Gill, and Dr. Mark Seville to develop peptide tags and molecular biology products based upon the extraordinary affinity of biotin for avidin or streptavidin. While working at Affymax as a researcher, Millard was involved in the discovery of the AviTag technology, saw its commercial potential, and obtained the exclusive rights from Affymax. He started Avidity as a reagent company that sublicenses the right to use the AviTag technology to other companies and sells products for use with the technology.

AviTag History

AviTag is a patented biotin-tagging technology discovered at Affymax by Dr. Peter Schatz from a Directed Evolution approach called "Peptides-on-PlasmIDS." The Directed Evolution approach identified a number of peptide sequences that can be biotinylated by the BirA enzyme of E. coli, and the best of these sequences was called the AviTag. E. coli enzyme BirA biotinylates the 15 amino acid AviTag twice as fast as its natural substrate, the Biotin Carboxyl Carrier Protein (BCCP).

The enzymatic biotinylation of proteins using AviTag technology is preferred over chemical biotinylation of proteins because BirA enzyme gently, and highly specifically, labels the lysine residue of the AviTag. Prior to the AviTag, the smallest biotin-accepting domain was comprised of 75 amino acids.

The Future

As Avidity matures, the company is expanding its scientific horizons and investigating the use of bioluminescence—such as found in deep sea organisms—to make its products even more useful to the scientific community. Avidity was approached by Dr. Bruce Bryan of Prolume, Ltd., in 2004 to use the AviTag in conjunction with Prolume's Gaussia luciferase and Green Fluorescent Proteins (GFP). The first Gaussia-AviTag product was released in November, 2007.

Additionally, Avidity is working with several companies to develop a new generation of highly mobile, simple, inexpensive and incredibly powerful diagnostics by combining an extraordinarily sensitive optical sensor with luciferase-based assays. In conjunction with BP Proteomics (developer of novel assay biochemistry), Prolume (expertise in luminescent/fluorescent molecules and hardware innovator), Black Forest Engineering (optical device engineering), Avidity is developing protein and DNA microarray biochips and Biosensors for the detection and identification of disease pathogens and multiplexed High Throughput Screening (HTS).

Quality Guarantee & Customer Service

Avidity stands behind its products. If for any reason our product does not meet our customers expectations, we will replace the product at no cost or refund the purchase price of the product. Customers must notify Avidity within 90 days of purchase regarding any product issues.

Avidity is very responsive to all customer inquiries and strives to be a valued scientific partner by providing timely shipping of orders, expert technical support and the sharing of ideas.

Environmental Responsibility

Avidity is a concerned member of the global scientific community and has adopted corporate policies aimed to help combat global warming and environmental pollution and do its save the planet from humankind's destructive nature. Each year, we pledge a 1 of our net profits to be donated to non-profit environmental protection organizations. If you know of a worthy organization that should be considered to be a recipient of this corporate support, please do not hesitate to forward Avidity the information.

Partnerships and Collaborations

As scientists, we understand the value of partnering with both academic and for-profit research institutions. Avidity has opened its doors and labs to graduate students and post-doctoral fellows from the University of Colorado and the University of Denver, and we are always interested in scientific collaborations with other companies and academic institutions.


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