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產品名稱：重組大鼠巨噬細胞衍生的趨化因子/ CCL22蛋白
產品型號：rRtMDC/CCL22
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組大鼠巨噬細胞衍生的趨化因子/ CCL22蛋白與其它公司提供的重組蛋白不同，rRtMDC/CCL22蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠巨噬細胞衍生的趨化因子/ CCL22蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組大鼠巨噬細胞衍生的趨化因子/ CCL22蛋白

Synonyms STCP-1, MDC (1-69), Macrophage-derived Chemokine

Species Rat

Source Escherichia coli.

Molecular Weight Approximately 7.9 kDa, a single, non-glycosylated polypeptide chain containing 68 amino acids.

Quantity 5μg/20μg/1000μg

AA Sequence 重組大鼠巨噬細胞衍生的趨化因子/ CCL22蛋白GPYGANVEDS ICCQDYIRHP LPPRFVKEFY WTSKSCRKPG VVLITIKNRD ICADPRMLWV KKILHKLA

Purity > 96 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 重組大鼠巨噬細胞衍生的趨化因子/ CCL22蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH7.4.

Endotoxin Less than 1 EU/μg of rRtMDC/CCL22 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage 重組大鼠巨噬細胞衍生的趨化因子/ CCL22蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組大鼠巨噬細胞衍生的趨化因子/ CCL22蛋白

SDS-PAGE

Reference 1. Nomiyama H, Imai T, Kusuda J, et al. 1998. Cytogenet Cell Genet, 81: 10-1.
2. Godiska R, Chantry D, Raport CJ, et al. 1997. J Exp Med, 185: 1595-604.
3. Yamashita UandKuroda E. 2002. Crit Rev Immunol, 22: 105-14.
4. Katou F, Ohtani H, Nakayama T, et al. 2001. Am J Pathol, 158: 1263-70.

Background CCL22 is a protein encoded by the CCL22 gene. It is highly expressed in macrophage, monocyte-derived dendritic cell and thymus, additionally, also detected in the tissues of thymus, lymph node and appendix. CCL22 can bind to CCR4, and is a chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer cells, but not for neutrophils, eosinophils, and resting T-lymphocytes. After secreted from monocyte-derived dendritic cells, the protein can be proteolytic cleaved into three forms: MDC (3-69), MDC (5-69), MDC (7-69).

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Synonyms	STCP-1, MDC (1-69), Macrophage-derived Chemokine
Species	Rat
Source	Escherichia coli.
Molecular Weight	Approximately 7.9 kDa, a single, non-glycosylated polypeptide chain containing 68 amino acids.
Quantity	5μg/20μg/1000μg
AA Sequence	重組大鼠巨噬細胞衍生的趨化因子/ CCL22蛋白GPYGANVEDS ICCQDYIRHP LPPRFVKEFY WTSKSCRKPG VVLITIKNRD ICADPRMLWV KKILHKLA
Purity	> 96 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	重組大鼠巨噬細胞衍生的趨化因子/ CCL22蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH7.4.
Endotoxin	Less than 1 EU/μg of rRtMDC/CCL22 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	重組大鼠巨噬細胞衍生的趨化因子/ CCL22蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組大鼠巨噬細胞衍生的趨化因子/ CCL22蛋白
SDS-PAGE
Reference	1. Nomiyama H, Imai T, Kusuda J, et al. 1998. Cytogenet Cell Genet, 81: 10-1. 2. Godiska R, Chantry D, Raport CJ, et al. 1997. J Exp Med, 185: 1595-604. 3. Yamashita UandKuroda E. 2002. Crit Rev Immunol, 22: 105-14. 4. Katou F, Ohtani H, Nakayama T, et al. 2001. Am J Pathol, 158: 1263-70.
Background	CCL22 is a protein encoded by the CCL22 gene. It is highly expressed in macrophage, monocyte-derived dendritic cell and thymus, additionally, also detected in the tissues of thymus, lymph node and appendix. CCL22 can bind to CCR4, and is a chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer cells, but not for neutrophils, eosinophils, and resting T-lymphocytes. After secreted from monocyte-derived dendritic cells, the protein can be proteolytic cleaved into three forms: MDC (3-69), MDC (5-69), MDC (7-69).

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產品名稱：重組大鼠巨噬細胞衍生的趨化因子/ CCL22蛋白