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產品名稱：重組大鼠白細胞介素-2，液體蛋白
產品型號：rRtIL-2, Liquid
產品廠商：KALANG
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重組大鼠白細胞介素-2，液體蛋白與其它公司提供的重組蛋白不同，rRtIL-2, Liquid蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠白細胞介素-2，液體蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

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重組大鼠白細胞介素-2，液體蛋白

Synonyms T-cell Growth Factor, CGF

Species Rat

Accession P17108

GeneID 116562

Source 重組大鼠白細胞介素-2，液體蛋白Escherichia coli.

Molecular Weight Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 135 amino acids.

Quantity 5μg/20μg/1000μg

AA Sequence APTSSPAKET QQHLEQLLLD LQVLLRGIDN YKNLKLPMML TFKFYLPKQA TELKHLQCLE NELGALQRVL DLTQSKSFHL EDAGNFISNI RVTVVKLKGS ENKFECQFDD EPATVVEFLR RWIAICQSII STMTQ

Purity 重組大鼠白細胞介素-2，液體蛋白> 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 10⁶ IU/mg.

Physical Appearance Sterile Colorless liquid.

Formulation A 0.2 μm filtered concentrated sterile solution in 10 mM sodium citrate, pH 4.0, 30 % glycerol.

Endotoxin Less than 1 EU/μg of rRtIL-2, Liquid as determined by LAL method.

Storage 重組大鼠白細胞介素-2，液體蛋白Store at -20 ~ -80 °C. Avoid repeated freeze/thaw cycles.

重組大鼠白細胞介素-2，液體蛋白

Reference 1. Ma, A., R. Koka, and P. Burkett. 2006. Annu Rev Immunol, 24: 657-79.
2. Taniguchi, T., H. Matsui, T. Fujita, et al. 1983. Nature, 302: 305-10.
3. Liparoto, S.F., D.G. Myszka, Z. Wu, et al. 2002. Biochemistry, 41: 2543-51.
4. Bodnar, A., E. Nizsaloczki, G. Mocsar, et al. 2008. Immunol Lett, 116: 117-25.
5. Mosmann, T.R., T. Yokota, R. Kastelein, et al. 1987. J Immunol, 138: 1813-6.

Background IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL2Rα, 75 kDa IL2Rβ, 64 kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes. Recombinant rat IL-2 is a 15.3 kDa protein containing 134 amino acid residues and it shares about 82 %, 66 %, 66 %, 60 % and 54 % amino acid sequence identity with mouse, human, rhesus macaque, pig and equus caballus IL-2, respectively.

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Synonyms	T-cell Growth Factor, CGF
Species	Rat
Accession	P17108
GeneID	116562
Source	重組大鼠白細胞介素-2，液體蛋白Escherichia coli.
Molecular Weight	Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 135 amino acids.
Quantity	5μg/20μg/1000μg
AA Sequence	APTSSPAKET QQHLEQLLLD LQVLLRGIDN YKNLKLPMML TFKFYLPKQA TELKHLQCLE NELGALQRVL DLTQSKSFHL EDAGNFISNI RVTVVKLKGS ENKFECQFDD EPATVVEFLR RWIAICQSII STMTQ
Purity	重組大鼠白細胞介素-2，液體蛋白> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 10⁶ IU/mg.
Physical Appearance	Sterile Colorless liquid.
Formulation	A 0.2 μm filtered concentrated sterile solution in 10 mM sodium citrate, pH 4.0, 30 % glycerol.
Endotoxin	Less than 1 EU/μg of rRtIL-2, Liquid as determined by LAL method.
Storage	重組大鼠白細胞介素-2，液體蛋白Store at -20 ~ -80 °C. Avoid repeated freeze/thaw cycles.
重組大鼠白細胞介素-2，液體蛋白
Reference	1. Ma, A., R. Koka, and P. Burkett. 2006. Annu Rev Immunol, 24: 657-79. 2. Taniguchi, T., H. Matsui, T. Fujita, et al. 1983. Nature, 302: 305-10. 3. Liparoto, S.F., D.G. Myszka, Z. Wu, et al. 2002. Biochemistry, 41: 2543-51. 4. Bodnar, A., E. Nizsaloczki, G. Mocsar, et al. 2008. Immunol Lett, 116: 117-25. 5. Mosmann, T.R., T. Yokota, R. Kastelein, et al. 1987. J Immunol, 138: 1813-6.
Background	IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL2Rα, 75 kDa IL2Rβ, 64 kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes. Recombinant rat IL-2 is a 15.3 kDa protein containing 134 amino acid residues and it shares about 82 %, 66 %, 66 %, 60 % and 54 % amino acid sequence identity with mouse, human, rhesus macaque, pig and equus caballus IL-2, respectively.

產品名稱：重組大鼠白細胞介素-2，液體蛋白