產品詳情
簡單介紹：
重組大鼠白細胞介素-7蛋白與其它公司提供的重組蛋白不同，rRtIL-7蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠白細胞介素-7蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組大鼠白細胞介素-7蛋白
| Species | Rat |
| Accession | P56478 |
| GeneID | 25647 |
| Source | Escherichia coli. |
| Molecular Weight | 重組大鼠白細胞介素-7蛋白Approximately 14. 9 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | DCHIKDKDGK AFGSVLMISI NQLDKMTGTD SDCPNNEPNF FKKHLCDDTK EAAFLNRAAR KLRQFLKMNI SEEFNDHLLR VSDGTQTLVN CTSKEEKTIK EQKKNDPCFL KRLLREIKTC WNKILKGSI |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組大鼠白細胞介素-7蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine 2E8 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rRtIL-7 as determined by LAL method. |
| Reconstitution | 重組大鼠白細胞介素-7蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組大鼠白細胞介素-7蛋白 | |
| SDS-PAGE |
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| Reference |
1. Goodwin RG, Lupton S, Schmierer A, et al. 1989. Proc Natl Acad Sci U S A. 86:302-6. 2. Sutherland GR, Baker E, Fernandez KE, et al. 1989. Hum Genet. 82:371-2. 3. Lupton SD, Gimpel S, Jerzy R, et al. 1990. J Immunol. 144:3592-601. 4. Noguchi M, Nakamura Y, Russell SM, et al. 1993. Science. 262:1877-80. 5. Fry TJ, Mackall CL. 2002. Blood. 99:3892-904. 6. Fry TJ, Mackall CL. 2002. J Hematother Stem Cell Res. 11:803-7. |
| Background | Interleukin-7 (IL-7) is encoded by the IL7 gene and secreted by stromal cells in the red marrow and thymus. It binds to the IL-7 receptor, a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells and also stimulates proliferation of B cells, T cells and NK cells. Furthermore, IL-7 as an immunotherapy agent has been examined in many human clinical trials for various malignancies and during HIV infection. Rat IL-7 contains 129 amino acid residues and has three disulfide bonds. In addition, it has approximately 57 % and 88 % amino acid sequence identity with human and murine IL-7. |
