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產品名稱：重組鼠成纖維細胞生長因子17蛋白
產品型號：rMuFGF-17
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組鼠成纖維細胞生長因子17蛋白與其它公司提供的重組蛋白不同，rMuFGF-17蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠成纖維細胞生長因子17蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組鼠成纖維細胞生長因子17蛋白

Species Murine

Source Escherichia coli.

Molecular Weight Approximately 22.5 kDa, a single non-glycosylated polypeptide chain containing 194 amino acids.

AA Sequence TQGENHPSPN FNQYVRDQGA MTDQLSRRQI REYQLYSRTS GKHVQVTGRR ISATAEDGNK FAKLIVETDT FGSRVRIKGA ESEKYICMNK RGKLIGKPSG KSKDCVFTEI VLENNYTAFQ NARHEGWFMA FTRQGRPRQA SRSRQNQREA HFIKRLYQGQ LPFPNHAERQ KQFEFVGSAP TRRTKRTRRP QSQT

Purity > 98 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組鼠成纖維細胞生長因子17蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 10⁵ IU/mg in the presence of 10 μg/ml of heparin.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 700 mM NaCl, with 0.02 % Tween-20.

Endotoxin Less than 0.1 EU/μg of rMuFGF-17 as determined by LAL method.

Reconstitution 重組鼠成纖維細胞生長因子17蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage 重組鼠成纖維細胞生長因子17蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組鼠成纖維細胞生長因子17蛋白

Background FGF-17 is a member of the FGF superfamily of heparin-binding mitogenic molecules characterized by the presence of a core, 120 amino acid (aa) beta-trefoil structure. The mRNA of FGF-17 was found in midgestation of embryo and multiple ***** tissues, and is preferentially expressed in specific sites, such as embryonic brain, developing skeleton and arteries.

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Species	Murine
Source	Escherichia coli.
Molecular Weight	Approximately 22.5 kDa, a single non-glycosylated polypeptide chain containing 194 amino acids.
AA Sequence	TQGENHPSPN FNQYVRDQGA MTDQLSRRQI REYQLYSRTS GKHVQVTGRR ISATAEDGNK FAKLIVETDT FGSRVRIKGA ESEKYICMNK RGKLIGKPSG KSKDCVFTEI VLENNYTAFQ NARHEGWFMA FTRQGRPRQA SRSRQNQREA HFIKRLYQGQ LPFPNHAERQ KQFEFVGSAP TRRTKRTRRP QSQT
Purity	> 98 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組鼠成纖維細胞生長因子17蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 10⁵ IU/mg in the presence of 10 μg/ml of heparin.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 700 mM NaCl, with 0.02 % Tween-20.
Endotoxin	Less than 0.1 EU/μg of rMuFGF-17 as determined by LAL method.
Reconstitution	重組鼠成纖維細胞生長因子17蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	重組鼠成纖維細胞生長因子17蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組鼠成纖維細胞生長因子17蛋白
Background	FGF-17 is a member of the FGF superfamily of heparin-binding mitogenic molecules characterized by the presence of a core, 120 amino acid (aa) beta-trefoil structure. The mRNA of FGF-17 was found in midgestation of embryo and multiple ***** tissues, and is preferentially expressed in specific sites, such as embryonic brain, developing skeleton and arteries.

產品名稱：重組鼠成纖維細胞生長因子17蛋白