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產(chǎn)品名稱：重組鼠白血病抑制因子蛋白
產(chǎn)品型號(hào)：rMuLIF
產(chǎn)品廠商：KALANG
產(chǎn)品文檔：

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重組鼠白血病抑制因子蛋白與其它公司提供的重組蛋白不同，rMuLIF蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對(duì)細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡(jiǎn)便高效，將蛋白純化過程中對(duì)蛋白的損傷降低到*小程度.重組鼠白血病抑制因子蛋白（全長(zhǎng)序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測(cè)、ELISA等試驗(yàn)中.

詳情介紹：

重組鼠白血病抑制因子蛋白

Synonyms Differentiation-stimulating Factor, D factor, Melanoma-derived LPL Inhibitor , MLPLI

Species Murine

Accession P09056

GeneID 16878

Source Escherichia coli.

Molecular Weight 重組鼠白血病抑制因子蛋白Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.

Quantity 5μg/25μg/1000μg

AA Sequence SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF

Purity > 98 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組鼠白血病抑制因子蛋白Fully biologically active when compared to standard. The specific activity is determined by inducing differentiation of murine M1 myeloid leukemic cells. The minimum detectable concentration of rMuLIF in this assay is 0.01 ng/ml. The specific activity of > 1.0 × 10⁸ IU/mg, where 50 units is defined as the amount of rMuLIF required to induce differentiation in 50 % of the M1 colonies in 1 ml agar cultures.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, with 0.02 % TWEEN 20.

Endotoxin Less than 1 EU/μg of rMuLIF as determined by LAL method.

Reconstitution 重組鼠白血病抑制因子蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組鼠白血病抑制因子蛋白

SDS-PAGE

Reference 1. Eswari S, Sai Kumar G, Sharma GT. 2012. Zygote: 1-11.
2. Mathieu ME, Saucourt C, Mournetas V, et al. 2012. Stem Cell Rev, 8: 1-15.
3. Thomson AJ, Pierart H, Meek S, et al. 2012. Cell Reprogram, 14: 112-22.
4. Li HandGrumet M. 2007. Glia, 55: 24-35.

Background Leukemia inhibitory factor (LIF) is a member of Interleukin 6 family. This protein is mainly expressed in the trophectoderm of the developing embryo, with its receptor LIFR expressed throughout the inner cell mass. LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. LIF is used in mouse embryonic stem cell culture, because that removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. It is also used in phase II clinical trial, which can assist embryo implantation in women who have failed to become pregnant despite assisted reproductive technologies (ART). Mature mouse LIF shares 78 % a.a. sequence identity with Human LIF.

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Synonyms	Differentiation-stimulating Factor, D factor, Melanoma-derived LPL Inhibitor , MLPLI
Species	Murine
Accession	P09056
GeneID	16878
Source	Escherichia coli.
Molecular Weight	重組鼠白血病抑制因子蛋白Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.
Quantity	5μg/25μg/1000μg
AA Sequence	SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF
Purity	> 98 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組鼠白血病抑制因子蛋白Fully biologically active when compared to standard. The specific activity is determined by inducing differentiation of murine M1 myeloid leukemic cells. The minimum detectable concentration of rMuLIF in this assay is 0.01 ng/ml. The specific activity of > 1.0 × 10⁸ IU/mg, where 50 units is defined as the amount of rMuLIF required to induce differentiation in 50 % of the M1 colonies in 1 ml agar cultures.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, with 0.02 % TWEEN 20.
Endotoxin	Less than 1 EU/μg of rMuLIF as determined by LAL method.
Reconstitution	重組鼠白血病抑制因子蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組鼠白血病抑制因子蛋白
SDS-PAGE
Reference	1. Eswari S, Sai Kumar G, Sharma GT. 2012. Zygote: 1-11. 2. Mathieu ME, Saucourt C, Mournetas V, et al. 2012. Stem Cell Rev, 8: 1-15. 3. Thomson AJ, Pierart H, Meek S, et al. 2012. Cell Reprogram, 14: 112-22. 4. Li HandGrumet M. 2007. Glia, 55: 24-35.
Background	Leukemia inhibitory factor (LIF) is a member of Interleukin 6 family. This protein is mainly expressed in the trophectoderm of the developing embryo, with its receptor LIFR expressed throughout the inner cell mass. LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. LIF is used in mouse embryonic stem cell culture, because that removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. It is also used in phase II clinical trial, which can assist embryo implantation in women who have failed to become pregnant despite assisted reproductive technologies (ART). Mature mouse LIF shares 78 % a.a. sequence identity with Human LIF.

產(chǎn)品名稱：重組鼠白血病抑制因子蛋白