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產品名稱：重組人白細胞介素-22蛋白
產品型號：rHuIL-22
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人白細胞介素-22蛋白與其它公司提供的重組蛋白不同，rHuIL-22蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人白細胞介素-22蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人白細胞介素-22蛋白

Synonyms Cytokine Zcyto 18, TIF

Species Human

Accession Q9GZX6

GeneID 50616

Source Escherichia coli.

Molecular Weight 重組人白細胞介素-22蛋白Approximately 33.6 kDa, non-disulfide-linked homodimeric protein containing of two 147 amino acid polypeptide chains.

Quantity 2μg/10μg/1000μg

AA Sequence MAPISSHCRL DKSNFQQPYI TNRTFMLAKE ASLADNNTDV RLIGEKLFHG VSMSERCYLM KQVLNFTLEE VLFPQSDRFQ PYMQEVVPFL ARLSNRLSTC HIEGDDLHIQ RNVQKLKDTV KKLGESGEIK AIGELDLLFM SLRNACI

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組人白細胞介素-22蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by inducing IL-10 secretion of human COLO 205 cells is less than 0.3 ng/ml, corresponding to a specific activity of > 3.3 × 10⁶ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 5.0.

Endotoxin Less than 1 EU/μg of rHuIL-22 as determined by LAL method.

Reconstitution 重組人白細胞介素-22蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人白細胞介素-22蛋白

Reference 1. Zeng G, Chen CY, Huang D, et al. 2011. J Immunol, 187: 190-9.
2. Cocco CandAiroldi I. 2012. Immunotherapy, 4: 668.
3. Honda K. 2012. Immunity, 37: 952-4.
4. Gurney AL. 2004. Int Immunopharmacol, 4: 669-77.

Background IL-22 is belonging to IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes and dendritic cells, IL-10 contributes to the inflammatory response in vivo. IL-22 signals through CRF2-4 and IL-22. It along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems.

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Synonyms	Cytokine Zcyto 18, TIF
Species	Human
Accession	Q9GZX6
GeneID	50616
Source	Escherichia coli.
Molecular Weight	重組人白細胞介素-22蛋白Approximately 33.6 kDa, non-disulfide-linked homodimeric protein containing of two 147 amino acid polypeptide chains.
Quantity	2μg/10μg/1000μg
AA Sequence	MAPISSHCRL DKSNFQQPYI TNRTFMLAKE ASLADNNTDV RLIGEKLFHG VSMSERCYLM KQVLNFTLEE VLFPQSDRFQ PYMQEVVPFL ARLSNRLSTC HIEGDDLHIQ RNVQKLKDTV KKLGESGEIK AIGELDLLFM SLRNACI
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組人白細胞介素-22蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by inducing IL-10 secretion of human COLO 205 cells is less than 0.3 ng/ml, corresponding to a specific activity of > 3.3 × 10⁶ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 5.0.
Endotoxin	Less than 1 EU/μg of rHuIL-22 as determined by LAL method.
Reconstitution	重組人白細胞介素-22蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人白細胞介素-22蛋白
Reference	1. Zeng G, Chen CY, Huang D, et al. 2011. J Immunol, 187: 190-9. 2. Cocco CandAiroldi I. 2012. Immunotherapy, 4: 668. 3. Honda K. 2012. Immunity, 37: 952-4. 4. Gurney AL. 2004. Int Immunopharmacol, 4: 669-77.
Background	IL-22 is belonging to IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes and dendritic cells, IL-10 contributes to the inflammatory response in vivo. IL-22 signals through CRF2-4 and IL-22. It along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems.

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產品名稱：重組人白細胞介素-22蛋白