產(chǎn)品詳情
簡單介紹：
重組大鼠β-防御素4蛋白與其它公司提供的重組蛋白不同，rRtBD-4蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠β-防御素4蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組大鼠β-防御素4蛋白
| Synonyms | Defensin beta4 |
| Species | Rat |
| Accession | O88514 |
| GeneID | 64389 |
| Source | Escherichia coli. |
| Molecular Weight | 重組大鼠β-防御素4蛋白Approximately 4.4 kDa, a single non-glycosylated polypeptide chain containing 41 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | QSINNPITCL TKGGVCWGPC TGGFRQIGTC GLPRVRCCKK K |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組大鼠β-防御素4蛋白Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 0.1-100.0 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, pH 7.4, 500 mM NaCl. |
| Endotoxin | 重組大鼠β-防御素4蛋白Less than 1 EU/μg of rRtBD-4 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組大鼠β-防御素4蛋白 | |
| SDS-PAGE |
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| Reference |
1. Musumeci G, Carnazza ML, Loreto C, et al. 2012. Acta Histochem, 114: 805-12. 2. Biragyn A, Ruffini PA, Leifer CA, et al. 2002. Science, 298: 1025-9. 3. Otte JM, Neumann HM, Brand S, et al. 2009. Eur J Clin Invest, 39: 126-38. |
| Background | Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. β-Defensins are expressed on some leukocytes and at epithelial surfaces. They contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Because β-defensins are cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane. Additionally, they are not only have the ability to strengthen the innate immune system but can also enhance the adaptive immune system by chemotaxis of monocytes, T-lymphocytes, dendritic cells and mast cells to the infection site. |
