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產(chǎn)品詳情
  • 產(chǎn)品名稱:重組大鼠白細(xì)胞介素3 beta蛋白

  • 產(chǎn)品型號:rRtIL-3β
  • 產(chǎn)品廠商:KALANG
  • 產(chǎn)品文檔:
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簡單介紹:
重組大鼠白細(xì)胞介素3 beta蛋白與其它公司提供的重組蛋白不同,rRtIL-3β蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白,可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡便高效,將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠白細(xì)胞介素3 beta蛋白(全長序列)產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測、ELISA等試驗(yàn)中.
詳情介紹:

重組大鼠白細(xì)胞介素3 beta蛋白

Synonyms Hematopoietic Growth Factor, MCGF, Multipotential Colony-stimulating Factor, P-cell-stimulating Factor.
Species Rat
Accession P97688
GeneID 24495
Source Escherichia coli.
Molecular Weight 重組大鼠白細(xì)胞介素3 beta蛋白Approximately 16.3 kDa, a single non-glycosylated polypeptide chain containing 144 amino acids.
Quantity 5μg/20μg/1000μg
AA Sequence MISDRGSDAH HLLRTLDCRT IALEILVKLP YPQVSGLNNS DDKANLRNST LRRVNLDEFL KSQEEFDSQD TTDIKSKLQK LKCCIPAAAS DSVLPGVYNK DLDDFKKKLR FYVIHLKDLQ PVSVSRPPQP TSSSDNFRPM TVEC
Purity > 95 % by SDS-PAGE and HPLC analyses.
Biological Activity 重組大鼠白細(xì)胞介素3 beta蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC-9 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin Less than 1 EU/μg of rRtIL-3β as determined by LAL method.
Reconstitution 重組大鼠白細(xì)胞介素3 beta蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組大鼠白細(xì)胞介素3 beta蛋白
SDS-PAGE
Reference 1. Yang YC, Ciarletta AB, Temple PA, et al. 1986. Cell. 47:3-10.
2. Otsuka T, Miyajima A, Brown N, et al. 1988. J Immunol. 140:2288-95.
3. Cohen DR, Hapel AJ, Young IG. 1986. Nucleic Acids Res. 14:3641-58.
4. Esandi MC, van Someren GD, Bout A, et al. 1998. Gene Ther. 5:778-88.
5. Dorssers L, Burger H, Bot F, et al. 1987. Gene. 55:115-24.
Background Interleukin-3 (IL-3) is a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. It exerts its biological activities through binding to Interleukin-3 receptors included α and β subunits, but sequence analysis revealed a number of features indicative of the presence of only one β-subunit in the rat. Interleukin-3 acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. Furthermore, rat and human IL-3 share low homology and have not cross species activity.

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