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  • 產品名稱:重組小鼠生長分化因子5 /骨形態發生蛋白-14蛋白

  • 產品型號:rMuGDF-5/BMP-14
  • 產品廠商:KALANG
  • 產品文檔:
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重組小鼠生長分化因子5 /骨形態發生蛋白-14蛋白與其它公司提供的重組蛋白不同,rMuGDF-5/BMP-14蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白,可表達出對細胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效,將蛋白純化過程中對蛋白的損傷降低到*小程度.重組小鼠生長分化因子5 /骨形態發生蛋白-14蛋白(全長序列)產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹:

重組小鼠生長分化因子5 /骨形態發生蛋白-14蛋白

Species Murine
Accession P43027
GeneID 14563
Source 重組小鼠生長分化因子5 /骨形態發生蛋白-14蛋白Escherichia coli.
Molecular Weight Approximately 27.2 kDa, a disulfide-linked homodimeric protein containing two 120 amino acids.
AA Sequence APLANRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR
Purity 重組小鼠生長分化因子5 /骨形態發生蛋白-14蛋白> 96 % by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Endotoxin Less than 0.1 EU/μg of rMuGDF-5/BMP-14 as determined by LAL method.
Reconstitution 重組小鼠生長分化因子5 /骨形態發生蛋白-14蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組小鼠生長分化因子5 /骨形態發生蛋白-14蛋白
Background Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.

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